| Long name: |
Biotin Conjugated, SRD5A1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SRD5A1 |
| Specificity: |
This is a highly specific antibody against SRD5A1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human SRD5A1 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
5a-Reductase 1 is expressed in all portions of the hair follicle, 5a-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5a-dihydrotestosterone, 5a-Reductase 1 shows heterogeneity of protein, In addition, In cultured human skin cells, The 5a-Reductase-3a-hydroxysteroid dehydrogenase complex is present in the human brain, The enzyme exists as two isoforms: type 1, and has different levels of transcriptional and translational expression, and type 2, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids, whereas 5a-Reductase 2 is expressed only in mesenchymal portions, which is expressed mainly in the prostate, which is expressed mainly in the skin, which mediates androgen effects on target tissues, Steroid 5a-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5a-dihydro-testosterone |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
SRD5A1 Conjugated |
| Short name: |
Conjugated, SRD5A1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, SRD5A1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11284 |
| Gene: |
SRD5A1 |
More about : SRD5A1 |
| Long gene name: |
steroid 5 alpha-reductase 1 |
| Synonyms gene name: |
alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) , steroid-5-alpha-reductase |
| Synonyms name: |
3-oxo-5-alpha-steroid 4-dehydrogenase 1 |
| Locus: |
5p15, 31 |
| Discovery year: |
1991-05-21 |
| GenBank acession: |
M32313 |
| Entrez gene record: |
6715 |
| Pubmed identfication: |
1686016 |
| RefSeq identity: |
NM_001047 |
| Havana BLAST/BLAT: |
OTTHUMG00000090456 |