| Long name: |
Biotin Conjugated, NSF Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
NSF |
| Specificity: |
This is a highly specific antibody against NSF |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human NSF |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Following membrane fusion, Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1, N-ethylmaleimide-sensitive factor (NSF), The ATPase activity of NSF induces a conformational change in the Î, a hexameric ATPase, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3, the Î, then associates with the Î, ±, ±, ±, -SNAP cytoplasmic adapter protein binds to the SNARE complex, -SNAP/SNARE complex that leads to its dissociation from the membrane, -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3, 2), 4), 4), Several protein-protein interactions are essential to membrane fusion during endocytosis |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
SKD2 SEC18 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
NSF Conjugated |
| Short name: |
Conjugated, NSF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, N-ethylmaleimide-sensitive factor (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ATPase activity, NSF and IDBG-55627 and ENSG00000073969 and 101930324, NSF and IDBG-641203 and ENSBTAG00000008442 and 504457, Nsf and IDBG-213066 and ENSMUSG00000034187 and 18195, Plasma membranes, SKD2, coupled, coupled and also this GO :0046872 : metal ion binding, coupled and molecular function this GO :0045026 and plasma membrane fusion and biological process this GO :0046872 and metal ion binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0001921 and positive regulation of receptor recycling and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006813 and potassium ion transport and biological process this GO :0006887 and exocytosis and biological process this GO :0007268 and synaptic transmission and biological process this GO :0015031 and protein transport and biological process this GO :0017075 and syntaxin-1 binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0019905 and syntaxin binding and molecular function this GO :0030165 and PDZ domain binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0042623 and ATPase activity, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0017075 : syntaxin-1 binding and also this GO :0019901 : protein kinase binding and also this GO :0019905 : syntaxin binding and also this GO :0030165 : PDZ domain binding and also this GO :0032403 : protein complex binding and also this GO :0042623 : ATPase activity, this GO :0005524 : ATP binding, this GO :0017075 : syntaxin-1 binding, this GO :0019901 : protein kinase binding, this GO :0019905 : syntaxin binding, this GO :0030165 : PDZ domain binding, this GO :0032403 : protein complex binding, this GO :0042623 : ATPase activity, this GO :0046872 : metal ion binding, 4905, N-ethylmaleimide-sensitive factor |
| Identity: |
8016 |
| Gene: |
NSF |
More about : NSF |
| Long gene name: |
N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| Synonyms gene name: |
N-ethylmaleimide-sensitive factor N-ethylmaleimide sensitive factor |
| Synonyms name: |
N-ethylmaleimide-sensitive factor-like protein |
| Locus: |
17q21, 31 |
| Discovery year: |
1996-08-28 |
| Entrez gene record: |
4905 |
| Pubmed identfication: |
1315316 8875895 |
| RefSeq identity: |
NM_006178 |
| Classification: |
AAA ATPases |
| Havana BLAST/BLAT: |
OTTHUMG00000134315 |