| Long name: |
Biotin Conjugated, AP2M1 (Thr156) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AP2M1 Thr156 |
| Specificity: |
This is a highly specific antibody against AP2M1 Thr156 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr156 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human AP2M1 around the phosphorylation site of Thr156 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
AP-1 and AP-2, AP-2m1, Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles, The Adaptin family, Two main types of adaptor proteins (APs), a 435 amino acid protein, a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1), and g classes, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, b, comprising a, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes, links Clathrin to receptors in coated vesicles, respectively, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis, Adaptins are heterotetrameric subunits of adaptors |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AP50 mu2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AP2M1 (Thr156) Conjugated |
| Short name: |
Conjugated, AP2M1 (Thr156) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, AP2M1 (Thr156) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
564 |
| Gene: |
AP2M1 |
More about : AP2M1 |
| Long gene name: |
adaptor related protein complex 2 mu 1 subunit |
| Synonyms gene: |
CLAPM1 |
| Synonyms gene name: |
adaptor-related protein complex 2, mu 1 subunit |
| Synonyms name: |
clathrin-associated/assembly/adaptor protein, medium 1 plasma membrane adaptor AP-2 50kDA protein clathrin coat adaptor protein AP50 clathrin adaptor complex AP2, mu subunit HA2 50 kDA subunit clathrin assembly protein complex 2 medium chain AP-2 mu 2 chain |
| Locus: |
3q27, 1 |
| Discovery year: |
2000-09-01 |
| GenBank acession: |
U36188 |
| Entrez gene record: |
1173 |
| Pubmed identfication: |
8595912 |
| RefSeq identity: |
NM_004068 |
| Havana BLAST/BLAT: |
OTTHUMG00000156822 |