| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
AP2M1 Thr156 |
| Specificity: |
This antibody reacts specifically with AP2M1 (Thr156) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr156 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human AP2M1 around the phosphorylation site of Thr156 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AP-1 and AP-2, AP-2m1, Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles, The Adaptin family, Two main types of adaptor proteins (APs), a 435 amino acid protein, a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1), and g classes, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, b, comprising a, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes, links Clathrin to receptors in coated vesicles, respectively, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis, Adaptins are heterotetrameric subunits of adaptors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AP50 mu2 |
| Also known as: |
AP2M1 (Thr156) Polyclonal Antibody |
| Other name: |
Anti-AP2M1 (Thr156) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
AP2M1 (Thr156) |
| Short name: |
Anti-AP2M1 (Thr156) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-AP2M1 (Thr156) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
564 |
| Gene: |
AP2M1 |
More about : AP2M1 |
| Long gene name: |
adaptor related protein complex 2 mu 1 subunit |
| Synonyms gene: |
CLAPM1 |
| Synonyms gene name: |
adaptor-related protein complex 2, mu 1 subunit |
| Synonyms name: |
clathrin-associated/assembly/adaptor protein, medium 1 plasma membrane adaptor AP-2 50kDA protein clathrin coat adaptor protein AP50 clathrin adaptor complex AP2, mu subunit HA2 50 kDA subunit clathrin assembly protein complex 2 medium chain AP-2 mu 2 chain |
| Locus: |
3q27, 1 |
| Discovery year: |
2000-09-01 |
| GenBank acession: |
U36188 |
| Entrez gene record: |
1173 |
| Pubmed identfication: |
8595912 |
| RefSeq identity: |
NM_004068 |
| Havana BLAST/BLAT: |
OTTHUMG00000156822 |