| Long name: |
FITC Conjugated, Sema3F/semaphorin 3F Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Sema3F/semaphorin 3F |
| Specificity: |
This is a highly specific antibody against Sema3F/semaphorin 3F |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
735-785/785 |
| Subcellular location: |
Secreted |
| Source: |
KLH conjugated synthetic peptide derived from human Sema3F |
| Gene ID number: |
6405 |
| Swiss Prot: |
Q13275 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
1993 [PubMed 8269517]), 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al, 1995 [PubMed7637782]), All the family members have a secretion signal, Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al, D, MIM 603961), SEMA3F is a secreted member of the semaphorin III family, Sequence comparisons have grouped the secreted semaphorins into 3 general classes, The semaphorin III family, a 500-amino acid sema domain, a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168), all have a basic domain at the C terminus, all of which also have an immunoglobulin domain, and 16 conserved cysteine residues(Kolodkin et al, and E, and mouse semaphorins A, chicken collapsin, consisting of human semaphorin III (SEMA3A, The semaphorins are a family of proteins that are involved in signaling |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SEMA-IV, SEMA3F, SEMAK, Sema III/F, Sema IV, Semaphorin IV, Semaphorin-3F, SEMA4 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Sema3F/semaphorin 3F Conjugated |
| Short name: |
FITC Conjugated, Sema3F/semaphorin 3F Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
(semaphorin) 3F/semaphorin 3F (Antibody to), fluorecein coupled, immunoglobulin domain (Ig), secreted, short basic domain, sema domain |
| Alternative technique: |
antibodies |