| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Sema3F/semaphorin 3F |
| Specificity: |
This antibody reacts specifically with Sema3F/semaphorin 3F |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Sema3F |
| Gene ID: |
6405 |
| Swiss Prot: |
Q13275 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
1993 [PubMed 8269517]), 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al, 1995 [PubMed7637782]), All the family members have a secretion signal, Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al, D, MIM 603961), SEMA3F is a secreted member of the semaphorin III family, Sequence comparisons have grouped the secreted semaphorins into 3 general classes, The semaphorin III family, a 500-amino acid sema domain, a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168), all have a basic domain at the C terminus, all of which also have an immunoglobulin domain, and 16 conserved cysteine residues(Kolodkin et al, and E, and mouse semaphorins A, chicken collapsin, consisting of human semaphorin III (SEMA3A, The semaphorins are a family of proteins that are involved in signaling |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SEMA-IV, SEMA3F, SEMAK, Sema III/F, Sema IV, Semaphorin IV, Semaphorin-3F, SEMA4 |
| Also known as: |
Sema3F/semaphorin 3F Polyclonal Antibody |
| Other name: |
Anti-Sema3F/semaphorin 3F Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Sema3F/semaphorin 3F |
| Short name: |
Anti-Sema3F/semaphorin 3F (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
(semaphorin) 3F/semaphorin 3F (polyclonal), ALEXA Fluor 594, immunoglobulin domain (Ig), secreted, short basic domain, antibody to-sema domain |
| Alternative technique: |
polyclonals |