| Long name: |
FITC Conjugated, SUFU/Suppressor of Fused Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SUFU/Suppressor of Fused |
| Specificity: |
This is a highly specific antibody against SUFU/Suppressor of Fused |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
440-484/484 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human SUFU |
| Gene ID number: |
51684 |
| Swiss Prot: |
Q9UMX1 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Down-regulates GLI1-mediated transactivation of target genes, GLI2 and GLI3 in the cytoplasm, GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state, May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome, Negative regulator of beta-catenin signaling, Part of a corepressor complex that acts on DNA-bound GLI1, Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL), Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity), SUFU dissociates from GLI3FL and the latter translocates to the nucleus, Sequesters GLI1, When Hh signaling is initiated, Without the Hh signal, and converted to a transcriptional activator (GLI3A), destabilized, leading to the efficient processing of GLI3FL into GLI3R, the SUFU-GLI3 complex is recruited to cilia, this effect is overcome by binding of STK36 to both SUFU and a GLI protein, where it is phosphorylated, Negative regulator in the hedgehog signaling pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
SUFUH SUFUXL PRO1280 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Description: |
HRAS like - suppressor protein make genetic suppression restoring the phenotype seen prior to the original background in , breast cancer tumor, Kinase, biological pathways |
| Gene target: |
SUFU/Suppressor of Fused Conjugated |
| Short name: |
FITC Conjugated, SUFU/Suppressor of Fused Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SUFU/Suppressor on Fused (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16466 |
| Gene: |
SUFU |
More about : SUFU |
| Long gene name: |
SUFU negative regulator of hedgehog signaling |
| Synonyms gene name: |
suppressor of fused homolog (Drosophila) |
| Locus: |
10q24, 32 |
| Discovery year: |
2001-08-28 |
| GenBank acession: |
AF175770 |
| Entrez gene record: |
51684 |
| Pubmed identfication: |
15367681 |
| RefSeq identity: |
NM_016169 |
| Havana BLAST/BLAT: |
OTTHUMG00000018966 |
| Locus Specific Databases: |
LRG_521 |