| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SUFU/Suppressor of Fused |
| Specificity: |
This antibody reacts specifically with SUFU/Suppressor of Fused |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SUFU |
| Gene ID: |
51684 |
| Swiss Prot: |
Q9UMX1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Down-regulates GLI1-mediated transactivation of target genes, GLI2 and GLI3 in the cytoplasm, GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state, May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome, Negative regulator of beta-catenin signaling, Part of a corepressor complex that acts on DNA-bound GLI1, Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL), Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity), SUFU dissociates from GLI3FL and the latter translocates to the nucleus, Sequesters GLI1, When Hh signaling is initiated, Without the Hh signal, and converted to a transcriptional activator (GLI3A), destabilized, leading to the efficient processing of GLI3FL into GLI3R, the SUFU-GLI3 complex is recruited to cilia, this effect is overcome by binding of STK36 to both SUFU and a GLI protein, where it is phosphorylated, Negative regulator in the hedgehog signaling pathway |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SUFUH SUFUXL PRO1280 |
| Also known as: |
SUFU/Suppressor of Fused Antibody |
| Other name: |
Anti-SUFU/Suppressor of Fused |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
HRAS like - suppressor protein make genetic suppression restoring the phenotype seen prior to the original background in , breast cancer tumor, Kinase, biological pathways |
| Gene target: |
SUFU/Suppressor of Fused |
| Short name: |
Fluor 594, Anti-SUFU/Suppressor of Fused |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SUFU/Suppressor on Fused |
| Identity: |
16466 |
| Gene: |
SUFU |
More about : SUFU |
| Long gene name: |
SUFU negative regulator of hedgehog signaling |
| Synonyms gene name: |
suppressor of fused homolog (Drosophila) |
| Locus: |
10q24, 32 |
| Discovery year: |
2001-08-28 |
| GenBank acession: |
AF175770 |
| Entrez gene record: |
51684 |
| Pubmed identfication: |
15367681 |
| RefSeq identity: |
NM_016169 |
| Havana BLAST/BLAT: |
OTTHUMG00000018966 |
| Locus Specific Databases: |
LRG_521 |