| Long name: |
HRP Conjugated, AMPK alpha-1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
HRP Conjugated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK alpha-1 |
| Specificity: |
This is a highly specific antibody against AMPK alpha-1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
5-30/559 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human AMPK alpha-1 |
| Gene ID number: |
5562 |
| Swiss Prot: |
Q13131 |
| Tested applications: |
WB |
| Recommended dilutions: |
WB(1:100-1000) |
| Crossreactivity: |
Monkey, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ACACB, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, EP300, FOXO3, GYS1, HDAC5, HMGCR and LIPE, HNF4A, In response to reduction of intracellular ATP levels, In response to stress, MEF2C, MLXIPL/ChREBP, PFKFB2 and PFKFB3, RPTOR and ATG1/ULK1: in response to nutrient limitation, Regulates insulin-signaling and glycolysis by phosphorylating IRS1, Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, SREBF1, SREBF2 and PPARGC1A, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, histone H2B, leading to CRTC2/TORC2 sequestration in the cytoplasm, leading to promote transcription, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2, p53/TP53, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), possibly by mediating phosphorylation of TBC1D4/AS160, probably by indirectly activating myosin, regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively, Catalytic subunit of AMP-activated protein kinase (AMPK) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% Gentamicin, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AMPK AMPKa2 |
| Properties: |
Also monoclonal HRP conjugates are often used for Western Blot, HRP conjugates are often primary rabbit polyclonal antibodies couples to the , found in the roots of , (HRP), C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°, enzyme , horseradish, horseradish peroxidase |
| Gene target: |
AMPK alpha-1 Conjugated |
| Short name: |
Conjugated, AMPK alpha-1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
HRP |
| Alternative name: |
horseradish peroxidase coupled, AMPK a-1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |