| Long name: |
Biotin Conjugated, AMPK alpha-1/2 (Thr183/Thr172) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AMPK alpha-1/2 Thr183/Thr172 |
| Specificity: |
This is a highly specific antibody against AMPK alpha-1/2 Thr183/Thr172 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr172/Thr183 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172 |
| Gene ID number: |
5562 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
AMPK is a cellular energy sensor conserved in all eukaryotic cells, AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation, Alternatively spliced transcript variants encoding distinct isoforms have been observed, It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK), It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways, The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio, [provided by RefSeq], The protein encoded by this gene belongs to the ser/thr protein kinase family |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AMPK AMPKa2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AMPK alpha-1/2 (Thr183/Thr172) Conjugated |
| Short name: |
Conjugated, AMPK alpha-1/2 (Thr183/Thr172) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, AMPK a-1/2 (Thr183/Thr172) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9377 |
| Gene: |
PRKAA2 |
More about : PRKAA2 |
| Long gene name: |
protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene: |
PRKAA |
| Synonyms gene name: |
AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Locus: |
1p32, 2 |
| Discovery year: |
1995-12-12 |
| GenBank acession: |
BC069823 |
| Entrez gene record: |
5563 |
| Pubmed identfication: |
7959015 |
| RefSeq identity: |
NM_006252 |
| Havana BLAST/BLAT: |
OTTHUMG00000008282 |