| Long name: |
FITC Conjugated, MLH1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MLH1 |
| Specificity: |
This is a highly specific antibody against MLH1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
520-570/756 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human MLH1 |
| Gene ID number: |
4292 |
| Swiss Prot: |
P40692 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also implicated in DNA damage signaling, Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2, DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected, DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis, It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch, MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, a component of the post-replicative DNA mismatch repair system (MMR), a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR, then MutL alpha is recruited to the heteroduplex, Heterodimerizes with PMS2 to form MutL alpha |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HNPCC FCC2 HNPCC2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MLH1 Conjugated |
| Short name: |
FITC Conjugated, MLH1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
coli) (Antibody to), colon cancer, fluorecein coupled, nonpolyposis classification 2 (E, mutL homolog 1 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, coli), colon cancer, nonpolyposis type 2 (E, nuclei, structure-specific DNA binding, this GO :0000289 and nuclear-transcribed mRNA poly(A) tail shortening and biological process this GO :0000712 and resolution of meiotic recombination intermediates and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0000794 and condensed nuclear chromosome and cellular component this GO :0000795 and synaptonemal complex and cellular component this GO :0001673 and male germ cell nucleus and cellular component this GO :0002204 and somatic recombination of immunoglobulin genes involved in immune response and biological process this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005694 and chromosome and cellular component this GO :0005712 and chiasma and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007060 and male meiosis chromosome segregation and biological process this GO :0007129 and synapsis and biological process this GO :0007131 and reciprocal meiotic recombination and biological process this GO :0007140 and male meiosis and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0016020 and membrane and cellular component this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0030983 and mismatched DNA binding and molecular function this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032300 and mismatch repair complex and cellular component this GO :0032389 and MutLalpha complex and cellular component this GO :0032407 and MutSalpha complex binding and molecular function this GO :0043060 and meiotic metaphase I plate congression and biological process this GO :0043566 and structure-specific DNA binding and molecular function this GO :0045132 and meiotic chromosome segregation and biological process this GO :0045190 and isotype switching and biological process this GO :0045950 and negative regulation of mitotic recombination and biological process this GO :0048477 and oogenesis and biological process this GO :0051257 and spindle midzone assembly involved in meiosis and biological process, this GO :0003697 : single-stranded DNA binding, this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016887 : ATPase activity and also this GO :0030983 : mismatched DNA binding and also this GO :0032137 : guanine/thymine mispair binding and also this GO :0032407 : MutSalpha complex binding and also this GO :0043566 : structure-specific DNA binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016887 : ATPase activity, this GO :0030983 : mismatched DNA binding, this GO :0032137 : guanine/thymine mispair binding, this GO :0032407 : MutSalpha complex binding, this GO :0043566 : structure-specific DNA binding, mutL homolog 1 |
| Identity: |
7127 |
| Gene: |
MLH1 |
More about : MLH1 |
| Long gene name: |
mutL homolog 1 |
| Synonyms gene: |
COCA2 |
| Synonyms gene name: |
coli) , coli) homolog 1 (colon cancer, colon cancer, mutL (E, nonpolyposis type 2 (E, nonpolyposis type 2) mutL homolog 1 |
| Locus: |
3p22, 2 |
| Discovery year: |
1993-11-24 |
| GenBank acession: |
U07418 |
| Entrez gene record: |
4292 |
| Pubmed identfication: |
7903889 |
| RefSeq identity: |
NM_000249 |
| Classification: |
MutL homologs |
| Havana BLAST/BLAT: |
OTTHUMG00000130797 |
| Locus Specific Databases: |
HNPCC Colon cancer gene variant databases MLH1 database at LOVD-China LOVD - Leiden Open Variation Database LRG_216 , Hereditary Non-Polyposis Colorectal Cancer |