| Long name: |
FITC Conjugated, HIF-1 Alpha Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HIF-1 Alpha |
| Specificity: |
This is a highly specific antibody against HIF-1 Alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
351-400/826 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha |
| Gene ID number: |
3091 |
| Swiss Prot: |
Q16665 |
| Tested applications: |
IF(ICC), IF(IHC-P) |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3, Activity is enhanced by interaction with both, Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters, HILPDA, Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP, Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia, NCOA1 or NCOA2, Plays an essential role in embryonic vascularization, Under hypoxic conditions, activates the transcription of over 4 genes, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia, glucose transporters, glycolytic enzymes, including erythropoietin, tumor angiogenesis and pathophysiology of ischemic disease, vascular endothelial growth factor, Functions as a master transcriptional regulator of the adaptive response to hypoxia |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HYPB HIF-1 KIAA1732 FLJ23184 KMT3A |
| Description: |
FITC Conjugated is a &alpha, - or alpha protein sometimes glycoprotein present in blood, The HIF-1 Alpha Antibody |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HIF-1 Alpha Conjugated |
| Short name: |
FITC Conjugated, HIF-1 Alpha Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HIF-1 a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
18420 |
| Gene: |
SETD2 |
More about : SETD2 |
| Long gene name: |
SET domain containing 2 |
| Locus: |
3p21, 31 |
| Discovery year: |
2006-02-15 |
| GenBank acession: |
AJ238403 |
| Entrez gene record: |
29072 |
| Pubmed identfication: |
16118227 11461154 |
| RefSeq identity: |
NM_014159 |
| Classification: |
Lysine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000133514 |
| Locus Specific Databases: |
LRG_775 |