| Long name: |
ALEXA FLUOR 488 Conjugated, HSP27 Polyclonal Antibody |
| Also known as: |
Anti-HSP27 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HSP27 |
| Specificity: |
This is a highly specific antibody against HSP27 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
100-150/205 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human HSP27 |
| Gene ID Number: |
3315 |
| Swiss Prot: |
P04792 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins, Akt (PKB), Another complex consists of HSP27 and the IKK complex, Cytoplasmic HSP27 exists in multiple complexes, HSP27, HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, MAPKAP-kinase 2, One complex consists of HSP27, Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, The presence of HSP27 in this complex is required for Akt activation by stress stimuli, along with other members of the small HSP group, and p38 MAPK, and stimulates HSP27 binding to the IKK complex, possesses a C-terminal Alpha-crystalline homology domain, thereby inhibiting filament extension, where it may function to stabilize DNA and/or the nuclear membrane, Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the HSP27 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
HSP27 |
| Short name: |
HSP27 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, HSP27 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5246 |
| Gene: |
HSPB1 |
More about : HSPB1 |
| Long gene name: |
heat shock protein family B (small) member 1 |
| Synonyms gene name: |
heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
X54079 |
| Entrez gene record: |
3315 |
| Pubmed identfication: |
2243808 9344682 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023228 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_248 |