| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HSP27 Ser15 |
| Specificity: |
This antibody reacts specifically with HSP27 (Ser15) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser15 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Ser15 |
| Gene ID: |
3315 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Because of the estrogen responsive nature of Hsp27, Hsp27, Hsp27 synthesis is induced by elevated temperature, Interestingly, MAP3K2/MEKK2, Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, This protein is involved in stress resistance and actin organization, This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors, [provided by RefSeq], also referred to as the Estrogen regulated 24K protein and HSP28, and MAP4K2/GCK, and environmental stresses, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, as well as estrogen in hormone responsive cells, but only one transcript variant has been supported and defined, endometrium and breast tissue, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein, is one of several small heat shock proteins (HSP) produced by all organisms studied, this protein has been studied extensively in human estrogen responsive tissues such as cervix, MAP3K3/MEKK5, The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Also known as: |
HSP27 (Ser15) Polyclonal Antibody |
| Other name: |
Anti-HSP27 (Ser15) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HSP27 (Ser15) |
| Short name: |
Anti-HSP27 (Ser15) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HSP27 (Ser15) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
5246 |
| Gene: |
HSPB1 |
More about : HSPB1 |
| Long gene name: |
heat shock protein family B (small) member 1 |
| Synonyms gene name: |
heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
X54079 |
| Entrez gene record: |
3315 |
| Pubmed identfication: |
2243808 9344682 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023228 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_248 |