| Long name: |
Cy3 Conjugated, Estrogen Receptor alpha Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Estrogen Receptor alpha |
| Specificity: |
This is a highly specific antibody against Estrogen Receptor alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
570-600/600 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from rat ER-alpha |
| Gene ID number: |
24890 |
| Swiss Prot: |
P06211 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Rat, Mouse |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ATF-2, Also mediates membrane-initiated estrogen signaling involving various kinase cascades, Can activate the transcriptional activity of TFF1, Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements, Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter, Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (By similarity), Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components, Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner, Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences, Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP, Sp1 and Sp3, The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues, c-Fos, such as AP-1/c-Jun, the function involves CREBBP, to mediate ERE-independent signaling, Nuclear hormone receptor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
ER, ER-alpha, Esr1, Estr, Estradiol receptor, Estrogen receptor, Nr3a1, Nuclear receptor subfamily 3 group A member 1, RNESTROR, Esr |
| Properties: |
1 of the Estrogen Receptor alpha Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Estrogen Receptor alpha Conjugated |
| Short name: |
Conjugated, Estrogen Receptor alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, Estrogen Receptor a (Antibody to) |
| Alternative technique: |
antibodies |