| Long name: |
FITC Conjugated, TRIM21 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
TRIM21 |
| Specificity: |
This is a highly specific antibody against TRIM21 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
100-150/475 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human TRIM21 |
| Gene ID number: |
6737 |
| Swiss Prot: |
P19474 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), At least two isoforms are present in nucleated and red blood cells, Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes, Enhances the decapping activity of DCP2, Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules, Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination, Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling, Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3, Plays a role in the regulation of the cell cycle progression, Promotes IRF8 ubiquitination, The common feature of these proteins is their ability to bind HY RNAs, UBE2D1, UBE2D2, UBE2E1 and UBE2E2, and tissue specific differences in RO/SSA proteins have been identified, thereby promoting its degradation by the proteasome, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway, 2, E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
RNF81 RO52 Ro/SSA |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
TRIM21 Conjugated |
| Short name: |
FITC Conjugated, TRIM21 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, TRIM21 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11312 |
| Gene: |
TRIM21 |
More about : TRIM21 |
| Long gene name: |
tripartite motif containing 21 |
| Synonyms gene: |
SSA1 |
| Synonyms gene name: |
Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) tripartite motif-containing 21 |
| Locus: |
11p15, 3 , 5-p15 |
| Discovery year: |
1994-01-06 |
| GenBank acession: |
AF391283 |
| Entrez gene record: |
6737 |
| Pubmed identfication: |
8094596 |
| RefSeq identity: |
NM_003141 |
| Classification: |
Tripartite motif containing Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000165701 |