| Long name: |
ALEXA FLUOR 488 Conjugated, TRIM21 Polyclonal Antibody |
| Also known as: |
Anti-TRIM21 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
TRIM21 |
| Specificity: |
This is a highly specific antibody against TRIM21 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
100-150/475 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human TRIM21 |
| Gene ID Number: |
6737 |
| Swiss Prot: |
P19474 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), At least two isoforms are present in nucleated and red blood cells, Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes, Enhances the decapping activity of DCP2, Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules, Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination, Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling, Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3, Plays a role in the regulation of the cell cycle progression, Promotes IRF8 ubiquitination, The common feature of these proteins is their ability to bind HY RNAs, UBE2D1, UBE2D2, UBE2E1 and UBE2E2, and tissue specific differences in RO/SSA proteins have been identified, thereby promoting its degradation by the proteasome, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway, 2, E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
RNF81 RO52 Ro/SSA |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the TRIM21 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
TRIM21 |
| Short name: |
TRIM21 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, TRIM21 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11312 |
| Gene: |
TRIM21 |
More about : TRIM21 |
| Long gene name: |
tripartite motif containing 21 |
| Synonyms gene: |
SSA1 |
| Synonyms gene name: |
Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) tripartite motif-containing 21 |
| Locus: |
11p15, 3 , 5-p15 |
| Discovery year: |
1994-01-06 |
| GenBank acession: |
AF391283 |
| Entrez gene record: |
6737 |
| Pubmed identfication: |
8094596 |
| RefSeq identity: |
NM_003141 |
| Classification: |
Tripartite motif containing Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000165701 |