| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TRIM21 |
| Specificity: |
This antibody reacts specifically with TRIM21 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human TRIM21 |
| Gene ID: |
6737 |
| Swiss Prot: |
P19474 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), At least two isoforms are present in nucleated and red blood cells, Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes, Enhances the decapping activity of DCP2, Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules, Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination, Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling, Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3, Plays a role in the regulation of the cell cycle progression, Promotes IRF8 ubiquitination, The common feature of these proteins is their ability to bind HY RNAs, UBE2D1, UBE2D2, UBE2E1 and UBE2E2, and tissue specific differences in RO/SSA proteins have been identified, thereby promoting its degradation by the proteasome, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway, 2, E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RNF81 RO52 Ro/SSA |
| Also known as: |
TRIM21 Polyclonal Antibody |
| Other name: |
Anti-TRIM21 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
TRIM21 |
| Short name: |
Anti-TRIM21 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TRIM21 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11312 |
| Gene: |
TRIM21 |
More about : TRIM21 |
| Long gene name: |
tripartite motif containing 21 |
| Synonyms gene: |
SSA1 |
| Synonyms gene name: |
Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) tripartite motif-containing 21 |
| Locus: |
11p15, 3 , 5-p15 |
| Discovery year: |
1994-01-06 |
| GenBank acession: |
AF391283 |
| Entrez gene record: |
6737 |
| Pubmed identfication: |
8094596 |
| RefSeq identity: |
NM_003141 |
| Classification: |
Tripartite motif containing Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000165701 |