| Long name: |
Biotin Conjugated, caspase-3 p12 subunit Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
caspase-3 p12 subunit |
| Specificity: |
This is a highly specific antibody against caspase-3 p12 subunit |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit |
| Gene ID number: |
836 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
7 and 9), 9 and 10), Alternative splicing of this gene results in two transcript variants which encode the same protein, Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176, Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e, Caspase 3 is the most extensively studied apoptotic protein among caspase family members, Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, Caspases 6, Caspases 8, Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells), In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e, PARP and DFF), The active Caspase 3 proteolytically cleaves and activates other caspases (e, The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme, as well as relevant targets in the cells (e, commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts), which is associated with neuronal death in Alzheimer's disease, The caspase family of cysteine proteases play a key role in apoptosis, g, g, g, g |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
APOPAIN, Apopain, CASP-3, CASP3, CASP3_HUMAN, CPP32, CPP32B, Caspase 3 apoptosis related cysteine protease, Caspase-3, Caspase3, Cysteine protease CPP32, Human cysteine protease CPP32 isoform alpha mRNA complete cds, PARP cleavage protease, Protein Yama, SCA 1, SCA-1, SCA1, SREBP cleavage activity 1, SREBP cleavage activity 1, Yama, Caspase-3 subunit p12 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Description: |
CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways, Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation |
| Gene target: |
caspase-3 p12 subunit Conjugated |
| Short name: |
Conjugated, caspase-3 p12 subunit Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, caspase-3 p12 functionnal sequence (Antibody to) |
| Alternative technique: |
antibodies |