| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
caspase-3 p12 subunit |
| Specificity: |
This antibody reacts specifically with caspase-3 p12 subunit |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human caspase-3 p12 subunit |
| Gene ID: |
836 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
7 and 9), 9 and 10), Alternative splicing of this gene results in two transcript variants which encode the same protein, Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176, Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e, Caspase 3 is the most extensively studied apoptotic protein among caspase family members, Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, Caspases 6, Caspases 8, Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells), In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e, PARP and DFF), The active Caspase 3 proteolytically cleaves and activates other caspases (e, The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme, as well as relevant targets in the cells (e, commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts), which is associated with neuronal death in Alzheimer's disease, The caspase family of cysteine proteases play a key role in apoptosis, g, g, g, g |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
APOPAIN, Apopain, CASP-3, CASP3, CASP3_HUMAN, CPP32, CPP32B, Caspase 3 apoptosis related cysteine protease, Caspase-3, Caspase3, Cysteine protease CPP32, Human cysteine protease CPP32 isoform alpha mRNA complete cds, PARP cleavage protease, Protein Yama, SCA 1, SCA-1, SCA1, SREBP cleavage activity 1, SREBP cleavage activity 1, Yama, Caspase-3 subunit p12 |
| Also known as: |
caspase-3 p12 subunit Antibody |
| Other name: |
Anti-caspase-3 p12 subunit |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways, Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation |
| Gene target: |
caspase-3 p12 subunit |
| Short name: |
Fluor 594, Anti-caspase-3 p12 subunit |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-caspase-3 p12 functionnal sequence |