| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Alkaline Phosphatase is produced by our Mammalian expression system and the target gene encoding Leu18-Ser502 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
5 kD, 54 |
| UniProt number: |
P05186 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry Ice/ice packs |
| Formulation: |
0, 150 mM sodium chloride, 2 mM MgSO4, pH 7, 1 mM ZnCl2, 2 um filtered solution of 20 mM HEPES, 5, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Avoid mixing using vortex or pipettes, Dissolve the protein in ddH2O, In order to retain the quality and the affinity of the product unchanged, It is not recommended to dilute the product to less than 100 &mu, avoid cycles of freezing and thawing, please, The vials should be centrifuged before it is opened, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
LVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
ALPL (C-6His), Alkaline Phosphatase |
| Short name: |
ALPL (C-6His), Recombinant Alkaline Phosphatase |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Label: |
Alkaline Phosphatase |
| Species: |
Humans, Human |
| Alternative name: |
alkaline phosphatase, liver/bone/kidney (C-6His), sapiens Alkaline Phosphatase, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994, ALPL and IDBG-93194 and ENSG00000162551 and 249, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, Extracellular, liver/bone/kidney, metal ion binding, this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001958 and endochondral ossification and biological process this GO :0003006 and developmental process involved in reproduction and biological process this GO :0003824 and catalytic activity and molecular function this GO :0004035 and alkaline phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0008152 and metabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016462 and pyrophosphatase activity and molecular function this GO :0016791 and phosphatase activity and molecular function this GO :0031012 and extracellular matrix and cellular component this GO :0031225 and anchored component of membrane and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033280 and response to vitamin D and biological process this GO :0046677 and response to antibiotic and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051384 and response to glucocorticoid and biological process this GO :0065010 and extracellular membrane-bounded organelle and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071529 and cementum mineralization and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004035 : alkaline phosphatase activity and also this GO :0005515 : protein binding and also this GO :0016462 : pyrophosphatase activity and also this GO :0016791 : phosphatase activity and also this GO :0046872 : metal ion binding, this GO :0004035 : alkaline phosphatase activity, this GO :0005515 : protein binding, this GO :0016462 : pyrophosphatase activity, this GO :0016791 : phosphatase activity, this GO :0046872 : metal ion binding, alkaline phosphatase |
| Identity: |
438 |
| Gene: |
ALPL |
More about : ALPL |
| Long gene name: |
alkaline phosphatase, liver/bone/kidney |
| Synonyms gene: |
HOPS |
| Synonyms: |
TNSALP |
| Locus: |
1p36, 12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
BC021289 |
| Entrez gene record: |
249 |
| Pubmed identfication: |
3532105 3446011 |
| RefSeq identity: |
NM_000478 |
| Classification: |
Alkaline phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000002949 |
| Locus Specific Databases: |
Tissue Nonspecific Alkaline Phosphatase Gene Mutation Database |