| Antigen detectable with this ELISA kit: |
Liver/Bone/Kidney (ALPL), Alkaline Phosphatase |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
28ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Liver/Bone/Kidney (ALPL), Liver/Bone/Kidney (ALPL), Liver/Bone/Kidney (ALPL), Liver/Bone/Kidney (ALPL) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, The concentration of Alkaline Phosphatase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Alkaline Phosphatase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Liver/Bone/Kidney (ALPL), Alkaline Phosphatase |
| Short name: |
Liver/Bone/Kidney (ALPL) ELISA kit, Alkaline Phosphatase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Label: |
Alkaline Phosphatase |
| Alternative name: |
Liver/Bone/Kidney (alkaline phosphatase, liver/bone/kidney) Enzyme-linked immunosorbent assay reagent, Alkaline Phosphatase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994, ALPL and IDBG-93194 and ENSG00000162551 and 249, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, Extracellular, liver/bone/kidney, metal ion binding, this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001958 and endochondral ossification and biological process this GO :0003006 and developmental process involved in reproduction and biological process this GO :0003824 and catalytic activity and molecular function this GO :0004035 and alkaline phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0008152 and metabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016462 and pyrophosphatase activity and molecular function this GO :0016791 and phosphatase activity and molecular function this GO :0031012 and extracellular matrix and cellular component this GO :0031225 and anchored component of membrane and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033280 and response to vitamin D and biological process this GO :0046677 and response to antibiotic and biological process this GO :0046872 and metal ion binding and molecular function this GO :0051384 and response to glucocorticoid and biological process this GO :0065010 and extracellular membrane-bounded organelle and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071529 and cementum mineralization and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004035 : alkaline phosphatase activity and also this GO :0005515 : protein binding and also this GO :0016462 : pyrophosphatase activity and also this GO :0016791 : phosphatase activity and also this GO :0046872 : metal ion binding, this GO :0004035 : alkaline phosphatase activity, this GO :0005515 : protein binding, this GO :0016462 : pyrophosphatase activity, this GO :0016791 : phosphatase activity, this GO :0046872 : metal ion binding, alkaline phosphatase |
| Identity: |
438 |
| Gene: |
ALPL |
More about : ALPL |
| Long gene name: |
alkaline phosphatase, liver/bone/kidney |
| Synonyms gene: |
HOPS |
| Synonyms: |
TNSALP |
| Locus: |
1p36, 12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
BC021289 |
| Entrez gene record: |
249 |
| Pubmed identfication: |
3532105 3446011 |
| RefSeq identity: |
NM_000478 |
| Classification: |
Alkaline phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000002949 |
| Locus Specific Databases: |
Tissue Nonspecific Alkaline Phosphatase Gene Mutation Database |