| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Secreted Phosphoprotein 1 is produced by our Mammalian expression system and the target gene encoding Ile17-Asn314 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
34, 75 kD |
| UniProt number: |
P10451 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
150 mM sodium chloride, pH 7, 2, 2 um filtered solution of 20 mM PB, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Avoid mixing using vortex or pipettes, Dissolve the protein in ddH2O, In order to retain the quality and the affinity of the product unchanged, It is not recommended to dilute the product to less than 100 &mu, avoid cycles of freezing and thawing, please, The vials should be centrifuged before it is opened, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVNVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
SPP1 SPP1 (C-6His), Secreted Phosphoprotein 1 |
| Short name: |
SPP1 SPP1 (C-6His), Recombinant Secreted Phosphoprotein 1 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
sapiens Secreted Phosphoprotein 1, secreted phosphoprotein 1 , secreted phosphoprotein 1 (C-6His), recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
BNSP and BSPI and ETA-1 and OPN, Extracellular, SPP1 and IDBG-29322 and ENSG00000118785 and 6696, SPP1 and IDBG-641200 and ENSBTAG00000005260 and 281499, Spp1 and IDBG-185485 and ENSMUSG00000029304 and 20750, extracellular matrix binding, this GO :0001503 and ossification and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007566 and embryo implantation and biological process this GO :0010033 and response to organic substance and biological process this GO :0010811 and positive regulation of cell-substrate adhesion and biological process this GO :0022617 and extracellular matrix disassembly and biological process this GO :0030154 and cell differentiation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0031214 and biomineral tissue development and biological process this GO :0031988 and membrane-bounded vesicle and cellular component this GO :0033280 and response to vitamin D and biological process this GO :0042995 and cell projection and cellular component this GO :0045087 and innate immune response and biological process this GO :0045177 and apical part of cell and cellular component this GO :0045780 and positive regulation of bone resorption and biological process this GO :0046697 and decidualization and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048545 and response to steroid hormone and biological process this GO :0048685 and negative regulation of collateral sprouting of intact axon in response to injury and biological process this GO :0050840 and extracellular matrix binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0050840 : extracellular matrix binding, this GO :0005515 : protein binding, this GO :0050840 : extracellular matrix binding, secreted phosphoprotein 1 |
| Identity: |
11255 |
| Gene: |
SPP1 |
More about : SPP1 |
| Long gene name: |
secreted phosphoprotein 1 |
| Synonyms gene: |
BNSP OPN |
| Synonyms gene name: |
osteopontin bone sialoprotein I |
| Synonyms: |
BSPI ETA-1 |
| Synonyms name: |
early T-lymphocyte activation 1 |
| Locus: |
4q22, 1 |
| Discovery year: |
1989-03-06 |
| Entrez gene record: |
6696 |
| Pubmed identfication: |
1575754 |
| Classification: |
Endogenous ligands SIBLING family |
| Havana BLAST/BLAT: |
OTTHUMG00000130599 |