| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, Antibody in 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PKN1 (C-Terminal) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
Western blot: 1:2000 |
| Notes: |
Titration of the PKN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This PKN1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16512 |
| Purity: |
Antigen affinity |
| Description: |
'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, 'Ser-637' and 'Ser-669' of MAPT/Tau, Able to phosphorylate RPS6 in vitro, Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, HDAC7 and HDAC9, NEFL and NEFM, Phosphorylates 'Ser-575', Phosphorylates 'Thr-38' of PPP1R14A, Phosphorylates HDAC5, Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C, and GFAP, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), cell migration, leading to impair their import in the nucleus, leading to inhibit their polymerization, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly, tumor cell invasion and transcription regulation, PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton |
| Immunogen: |
Amino acids 836-870 from the human protein were used as the immunogen for this PKN1 antibody |
| Storage: |
Avoid repeated freeze-thaw cycles, Aliquot the PKN1 antibody and store frozen at -20oC or colder |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PKN1 |
| Short name: |
PKN1 Antibody (C-Terminal) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
protein kinase N1 (Antibody to) (C-Terminal) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DBK and PAK-1 and PAK1 and PKN and PKN-ALPHA and PRK1 and PRKCL1, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006972 and hyperosmotic response and biological process this GO :0007165 and signal transduction and biological process this GO :0007257 and activation of JUN kinase activity and biological process this GO :0010631 and epithelial cell migration and biological process this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016772 and transferase activity, PKN1 and IDBG-33268 and ENSG00000123143 and 5585, PKN1 and IDBG-642677 and ENSBTAG00000017037 and 509080, Pkn1 and IDBG-172377 and ENSMUSG00000057672 and 320795, nuclei, this GO :0003682 and chromatin binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004697 and protein kinase C activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005768 and endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0003682 : chromatin binding, this GO :0003682 : chromatin binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004697 : protein kinase C activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005080 : protein kinase C binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004697 : protein kinase C activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005080 : protein kinase C binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0017049 : GTP-Rho binding, this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity, this GO :0035402 : histone kinase activity (H3-T11 specific), this GO :0042393 : histone binding, this GO :0042826 : histone deacetylase binding, this GO :0048365 : Rac GTPase binding, this GO :0050681 : androgen receptor binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0017049 : GTP-Rho binding and also this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity and also this GO :0035402 : histone kinase activity (H3-T11 specific) and also this GO :0042393 : histone binding and also this GO :0042826 : histone deacetylase binding and also this GO :0048365 : Rac GTPase binding and also this GO :0050681 : androgen receptor binding, transferring phosphorus-containing groups and molecular function this GO :0017049 and GTP-Rho binding and molecular function this GO :0030374 and ligand-dependent nuclear receptor transcription coactivator activity and molecular function this GO :0030496 and midbody and cellular component this GO :0032154 and cleavage furrow and cellular component this GO :0035402 and histone kinase activity (H3-T11 specific) and molecular function this GO :0035407 and histone H3-T11 phosphorylation and biological process this GO :0042393 and histone binding and molecular function this GO :0042826 and histone deacetylase binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0048365 and Rac GTPase binding and molecular function this GO :0050681 and androgen receptor binding and molecular function this GO :2000145 and regulation of cell motility and biological process, protein kinase N1 |
| Identity: |
9405 |
| Gene: |
PKN1 |
More about : PKN1 |
| Long gene name: |
protein kinase N1 |
| Synonyms gene: |
PRKCL1 |
| Synonyms gene name: |
protein kinase C-like 1 |
| Synonyms: |
DBK PRK1 PKN MGC46204 PAK1 |
| Locus: |
19p13, 12 |
| Discovery year: |
1997-10-10 |
| GenBank acession: |
S75546 |
| Entrez gene record: |
5585 |
| Pubmed identfication: |
9570957 |
| RefSeq identity: |
NM_002741 NM_213560 |
| Classification: |
C2 domain containing protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000039611 |