| Antigen detectable with this ELISA kit: |
Protein Kinase N1 (PKN1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1), The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Protein Kinase N1 (PKN1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Protein Kinase N1 (PKN1) |
| Short name: |
Protein Kinase N1 (PKN1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Protein phosphorylation catalyst N1 (protein kinase N1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DBK and PAK-1 and PAK1 and PKN and PKN-ALPHA and PRK1 and PRKCL1, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006972 and hyperosmotic response and biological process this GO :0007165 and signal transduction and biological process this GO :0007257 and activation of JUN kinase activity and biological process this GO :0010631 and epithelial cell migration and biological process this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016772 and transferase activity, PKN1 and IDBG-33268 and ENSG00000123143 and 5585, PKN1 and IDBG-642677 and ENSBTAG00000017037 and 509080, Pkn1 and IDBG-172377 and ENSMUSG00000057672 and 320795, nuclei, this GO :0003682 and chromatin binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004697 and protein kinase C activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005768 and endosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0003682 : chromatin binding, this GO :0003682 : chromatin binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004697 : protein kinase C activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005080 : protein kinase C binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004697 : protein kinase C activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005080 : protein kinase C binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0017049 : GTP-Rho binding, this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity, this GO :0035402 : histone kinase activity (H3-T11 specific), this GO :0042393 : histone binding, this GO :0042826 : histone deacetylase binding, this GO :0048365 : Rac GTPase binding, this GO :0050681 : androgen receptor binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0017049 : GTP-Rho binding and also this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity and also this GO :0035402 : histone kinase activity (H3-T11 specific) and also this GO :0042393 : histone binding and also this GO :0042826 : histone deacetylase binding and also this GO :0048365 : Rac GTPase binding and also this GO :0050681 : androgen receptor binding, transferring phosphorus-containing groups and molecular function this GO :0017049 and GTP-Rho binding and molecular function this GO :0030374 and ligand-dependent nuclear receptor transcription coactivator activity and molecular function this GO :0030496 and midbody and cellular component this GO :0032154 and cleavage furrow and cellular component this GO :0035402 and histone kinase activity (H3-T11 specific) and molecular function this GO :0035407 and histone H3-T11 phosphorylation and biological process this GO :0042393 and histone binding and molecular function this GO :0042826 and histone deacetylase binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0048365 and Rac GTPase binding and molecular function this GO :0050681 and androgen receptor binding and molecular function this GO :2000145 and regulation of cell motility and biological process, protein kinase N1 |
| Identity: |
9405 |
| Gene: |
PKN1 |
More about : PKN1 |
| Long gene name: |
protein kinase N1 |
| Synonyms gene: |
PRKCL1 |
| Synonyms gene name: |
protein kinase C-like 1 |
| Synonyms: |
DBK PRK1 PKN MGC46204 PAK1 |
| Locus: |
19p13, 12 |
| Discovery year: |
1997-10-10 |
| GenBank acession: |
S75546 |
| Entrez gene record: |
5585 |
| Pubmed identfication: |
9570957 |
| RefSeq identity: |
NM_002741 NM_213560 |
| Classification: |
C2 domain containing protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000039611 |