| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-ErbB2 (pY1221) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:2000-4000 |
| Notes: |
Titration of the phospho-ErbB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-ErbB2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P04626 |
| Purity: |
Antigen affinity |
| Description: |
Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription, Essential component of a neuregulin-receptor complex, GP30 is a potential ligand for this receptor, Implicated in transcriptional activation of CDKN1A, In the nucleus is involved in transcriptional regulation, In turn, Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth, Regulates outgrowth and stabilization of peripheral microtubules (MTs), This prevents the phosphorylation of APC and CLASP2, Upon ERBB2 activation, allowing its association with the cell membrane, although neuregulins do not interact with it alone, but that apparently needs a coreceptor for ligand binding, membrane-bound APC allows the localization of MACF1 to the cell membrane, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane, the function involves STAT3 and SRC, which is required for microtubule capture and stabilization, Protein tyrosine kinase that is part of several cell surface receptor complexes |
| Immunogen: |
This phospho-ErbB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1221 of human ErbB2 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-ErbB2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-ErbB2 (pY1221) |
| Short name: |
Phospho-ErbB2 Antibody (pY1221) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
neuro/glioblastoma derived oncogene homolog (avian) (Antibody to) (pY1221), phosphorilated-v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 |
| Alternative technique: |
antibodies |