| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PERK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the PERK antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This PERK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NZJ5 |
| Purity: |
Purified |
| Description: |
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), It likely serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1 Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, WRS is a rare autosomal recessive disorder, a member of the GCN2 subfamily of Ser/Thr protein kinases, also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus, at a later age, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, epiphyseal dysplasia, growth retardation and other multisystem manifestations, leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis, mental retardation and cardiovascular abnormalities, osteoporosis, phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), resulting in transautophosphorylation and kinase activity induction Expression of this Type I membrane protein is ubiquitous, such as hepatic and renal dysfunctions, with highest levels seen in secretory tissues, PERK |
| Immunogen: |
A portion of amino acids 148-175 from the human protein was used as the immunogen for this PERK antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PERK antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PERK |
| Short name: |
PERK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PERK (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3255 |
| Gene: |
EIF2AK3 |
More about : EIF2AK3 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 3 |
| Synonyms: |
PEK PERK |
| Locus: |
2p11, 2 |
| Discovery year: |
1999-06-14 |
| GenBank acession: |
AF110146 |
| Entrez gene record: |
9451 |
| Pubmed identfication: |
10026192 10575235 |
| RefSeq identity: |
NM_004836 |
| Havana BLAST/BLAT: |
OTTHUMG00000155046 |
| Locus Specific Databases: |
LRG_1024 |