| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PERK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the PERK antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This PERK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NZJ5 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization and radiation hybrid analysis, Harding et al, Hayes et al, Kittler et al, Using a library of endoribonuclease-prepared short interfering RNAs(esiRNAs), abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, also called PEK and PERK, is an enzyme encoded by the EIF2AK3 gene, one of which was PERK, resulting in abnormally elevated protein synthesis and higher levels of ER stress, which they called PERK, (1999) mapped the gene to chromosome 2p12, (2000) reported that a targeted mutation of the mouse Eif2ak3 gene, (2004) identified 37 genes required for cell division, Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3 |
| Immunogen: |
Amino acids 163-176 (QWDQDRESMETVPF) were used as the immunogen for this PERK antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PERK antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PERK |
| Short name: |
PERK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PERK (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3255 |
| Gene: |
EIF2AK3 |
More about : EIF2AK3 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 3 |
| Synonyms: |
PEK PERK |
| Locus: |
2p11, 2 |
| Discovery year: |
1999-06-14 |
| GenBank acession: |
AF110146 |
| Entrez gene record: |
9451 |
| Pubmed identfication: |
10026192 10575235 |
| RefSeq identity: |
NM_004836 |
| Havana BLAST/BLAT: |
OTTHUMG00000155046 |
| Locus Specific Databases: |
LRG_1024 |