| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
nNOS (phospho-S1417) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, DB |
| Recommended dilutions: |
Dot blot: 1:500 |
| Notes: |
Titration of the phospho-nNOS antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-nNOS antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29475 |
| Purity: |
Antigen affinity |
| Description: |
All are homodimers with subunits of 130-160 kDa, All have binding sites for NADPH, FAD, In response to increased intracellular Ca2+, NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways, NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO), Neuronal nitric oxide synthase (nNOS, Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production, The Ca2+ CaM complex, The dephosphorylation of nNOS by calcineurin initiates the production NO, The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain), bNOS, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm, cNOS, in combination with BH4, nNOS interacts with CaM, nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC), Three isoforms of nitric oxide synthase (NOS) have been identified |
| Immunogen: |
This phospho-nNOS antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1417 of human nNOS |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-nNOS antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
nNOS (phospho-S1417) |
| Short name: |
nNOS Antibody (phospho-S1417) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
nNOS (Antibody to) (phosphorilated-S1417) |
| Alternative technique: |
antibodies |