| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
nNOS |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the nNOS antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This nNOS antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29475 |
| Purity: |
Antigen affinity |
| Description: |
Despite this, Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length, It may be involved in neuronal cell death and damage in neurological illness, It splice variant, The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity, and playing a part in neuronal communication in the brain, expressed in testis, gene-knockout mice lacking neuronal(Type I) nitric oxide synthase exhibit relatively normal brain development and behavior, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2, nNOS1 localized to the 12q24, nNOS1) is a messenger molecule, neuronal NOS, that encodes an NH2-terminal truncated protein of 1098 amino acids, 2 region of human chromosome 12, Nitric Oxide Synthase 1(NOS1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of nNOS (IAFIEESKKDTDEVFSS) was used as the immunogen for this nNOS antibody (100% homologous human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the nNOS antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
nNOS |
| Short name: |
nNOS Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
nNOS (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7872 |
| Gene: |
NOS1 |
More about : NOS1 |
| Long gene name: |
nitric oxide synthase 1 |
| Synonyms gene: |
NOS |
| Synonyms gene name: |
nitric oxide synthase 1 (neuronal) |
| Synonyms: |
nNOS |
| Locus: |
12q24, 22 |
| Discovery year: |
1992-06-09 |
| Entrez gene record: |
4842 |
| Pubmed identfication: |
1385308 7682706 |
| Classification: |
PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000137376 |
| Locus Specific Databases: |
The Globin Gene Server |