| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
p70 S6 Kinase alpha (RPS6KB1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the p70S6K antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This p70S6K antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P23443 |
| Purity: |
Antigen affinity |
| Description: |
RPS6 and EEF2K, Regulates protein synthesis through phosphorylation of EIF4B, The active form then phosphorylates and activates several substrates in the pre-initiation complex, Under conditions of nutrient depletion, Upon mitogenic stimulation, [UniProt], and contributes to cell survival by repressing the pro-apoptotic function of BAD, cell growth and cell cycle progression, including the EIF2B complex and the cap-binding complex component EIF4B, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation, the inactive form associates with the EIF3 translation initiation complex, RPS6kB1 is a serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation |
| Immunogen: |
A portion of amino acids 425-454 from human RPS6KB1 was used as the immunogen for this p70S6K antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the p70S6K antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
p70 S6 Kinase alpha (RPS6KB1) |
| Short name: |
p70 S6 Kinase alpha Antibody (RPS6KB1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
70kDa, polypeptide 1), p70 S6 phosphorylation catalyst a (Antibody to) (ribosomal protein S6 phosphorylation catalyst |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
70kDa, RPS6KB1 and IDBG-61938 and ENSG00000108443 and 6198, RPS6KB1 and IDBG-629797 and ENSBTAG00000016851 and 404181, Rps6kb1 and IDBG-206895 and ENSMUSG00000020516 and 72508, nuclei, p70 S6KA and p70-alpha and p70-S6K and p70(S6K)-alpha and PS6K and S6K and S6K-beta-1 and S6K1 and STK14A, polypeptide 1, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0003009 and skeletal muscle contraction and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004711 and ribosomal protein S6 kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007165 and signal transduction and biological process this GO :0007281 and germ cell development and biological process this GO :0007568 and aging and biological process this GO :0007584 and response to nutrient and biological process this GO :0007616 and long-term memory and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0009408 and response to heat and biological process this GO :0009611 and response to wounding and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0009636 and response to toxic substance and biological process this GO :0009749 and response to glucose and biological process this GO :0009986 and cell surface and cellular component this GO :0010033 and response to organic substance and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014732 and skeletal muscle atrophy and biological process this GO :0014878 and response to electrical stimulus involved in regulation of muscle adaptation and biological process this GO :0014911 and positive regulation of smooth muscle cell migration and biological process this GO :0016477 and cell migration and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004711 : ribosomal protein S6 kinase activity and also this GO :0004712 : protein serine/threonine/tyrosine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004711 : ribosomal protein S6 kinase activity, this GO :0004712 : protein serine/threonine/tyrosine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0042277 : peptide binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0042277 : peptide binding, transferring phosphorus-containing groups and molecular function this GO :0030054 and cell junction and cellular component this GO :0031929 and TOR signaling and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032868 and response to insulin and biological process this GO :0032870 and cellular response to hormone stimulus and biological process this GO :0033574 and response to testosterone and biological process this GO :0033762 and response to gluca this GO n and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0042277 and peptide binding and molecular function this GO :0042493 and response to drug and biological process this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043200 and response to amino acid and biological process this GO :0043201 and response to leucine and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0045202 and synapse and cellular component this GO :0045471 and response to ethanol and biological process this GO :0045727 and positive regulation of translation and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0045948 and positive regulation of translational initiation and biological process this GO :0046324 and regulation of glucose import and biological process this GO :0046627 and negative regulation of insulin receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048633 and positive regulation of skeletal muscle tissue growth and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, ribosomal protein S6 kinase |
| Identity: |
10436 |
| Gene: |
RPS6KB1 |
More about : RPS6KB1 |
| Long gene name: |
ribosomal protein S6 kinase B1 |
| Synonyms gene: |
STK14A |
| Synonyms gene name: |
70kD, polypeptide 1 , ribosomal protein S6 kinase |
| Synonyms: |
S6K1 p70(S6K)-alpha PS6K |
| Locus: |
17q23, 1 |
| Discovery year: |
1994-07-11 |
| GenBank acession: |
M60724 |
| Entrez gene record: |
6198 |
| Pubmed identfication: |
1922062 |
| RefSeq identity: |
NM_003161 |
| Havana BLAST/BLAT: |
OTTHUMG00000150642 |