| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
p70 S6 kinase alpha (Rps6kb1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the S6k1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This S6k1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q8BSK8 |
| Purity: |
Antigen affinity |
| Description: |
Downstream effector of the mTOR signaling pathway, During translation initiation, Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B, Phosphorylates BAD at 'Ser-136' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis, Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity, Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance, Phosphorylates POLDIP3 (By similarity), Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling, Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex, Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens, Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation, Required for TNF-alpha induced IRS-1 degradation, The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function, cell cycle progression and cell survival, cell growth, cell proliferation, probably phosphorylates IRS1 at 'Ser-270', probably phosphorylates RICTOR at 'Thr-1135', probably phosphorylates RMP at 'Ser-136', the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion, Rps6kb1 acts to integrate nutrient and growth factor signals in regulation of protein synthesis |
| Immunogen: |
A portion of amino acids 481-509 from the mouse protein was used as the immunogen for this S6k1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the S6k1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
p70 S6 kinase alpha (Rps6kb1) |
| Short name: |
p70 S6 kinase alpha Antibody (Rps6kb1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
70kDa, polypeptide 1), p70 S6 phosphorylation catalyst a (Antibody to) (ribosomal protein S6 phosphorylation catalyst |
| Alternative technique: |
antibodies |