| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-p53 (pS315) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, DB |
| Recommended dilutions: |
Dot blot: 1:500, IHC (Paraffin): 1:50-1:100 |
| Notes: |
Titration of the phospho-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-p53 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P04637 |
| Purity: |
Antigen affinity |
| Description: |
Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, It is found in very low levels in normal cells, It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, a nuclear protein, and believed to contribute to transformation and malignancy, and hence cause the loss of tumor suppressor activity, and thus function as a tumor suppressor, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome, however, in a variety of transformed cell lines, it is expressed in high amounts, oligomerization and transcription activation domains, p53 is a DNA-binding protein containing DNA-binding, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1, Tumor protein p53 |
| Immunogen: |
This phospho-p53 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS315 of human p53 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-p53 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-p53 (pS315) |
| Short name: |
Phospho-p53 Antibody (pS315) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-p53 (Antibody to) (pS315) |
| Alternative technique: |
antibodies |