| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Phospho-IKKb (pY199) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the phospho-IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-IKKb antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O14920 |
| Purity: |
Antigen affinity |
| Description: |
Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs, Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, RELA, The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), or RELB to form the NFKB complex, thereby allowing activation of the NF-kappa-B complex, which inactivate NF-kappa-B by trapping it in the cytoplasm, NFKB1 or NFKB2 is bound to REL |
| Immunogen: |
This phospho-IKKb antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY199 of human IKKb |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-IKKb antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Phospho-IKKb (pY199) |
| Short name: |
Phospho-IKKb Antibody (pY199) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
phosphorilated-IKKb (Antibody to) (pY199) |
| Alternative technique: |
antibodies |