| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CHK1 (phospho-S280) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the p-CHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This p-CHK1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O14757 |
| Purity: |
Antigen affinity |
| Description: |
Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts, CDC25C, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, CHK1 phosphorylates and inhibits CDC25C, However, In response to DNA damage, In vitro, The S, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks, are completed with high fidelity, pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint, such as DNA replication and chromosome segregation, thus preventing activation of the CDC2-cyclin B complex and mitotic entry, CHK1 is modified in response to DNA damage, Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events |
| Immunogen: |
This p-CHK1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS280 of human CHK1 |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the p-CHK1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CHK1 (phospho-S280) |
| Short name: |
CHK1 Antibody (phospho-S280) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CHK1 (Antibody to) (phosphorilated-S280) |
| Alternative technique: |
antibodies |
| Identity: |
1925 |
| Gene: |
CHEK1 |
More about : CHEK1 |
| Long gene name: |
checkpoint kinase 1 |
| Synonyms gene name: |
CHK1 (checkpoint, S, pombe) , pombe) homolog CHK1 checkpoint homolog (S |
| Synonyms: |
CHK1 |
| Locus: |
11q24, 2 |
| Discovery year: |
1998-04-21 |
| GenBank acession: |
AF016582 BC017575 |
| Entrez gene record: |
1111 |
| Pubmed identfication: |
9278511 9382850 |
| RefSeq identity: |
NM_001274 |
| Havana BLAST/BLAT: |
OTTHUMG00000165853 |