| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Alkaline Phosphatase (liver) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, IHC, WB, FACS |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Alkaline Phosphatase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P05186 |
| Purity: |
Purified |
| Description: |
A proposed function of this form of the enzyme is matrix mineralization, The character of this disorder can vary, The exact physiological function of the alkaline phosphatases is not known, The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1, This enzyme has been linked directly to a disorder known as hypophosphatasia, This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, a disorder that is characterized by hypercalcemia and includes skeletal defects, and liver/bone/kidney (tissue non-specific), depending on the specific mutation since this determines age of onset and severity of symptoms, however, however, mice that lack a functional form of this enzyme show normal skeletal development, placental, placental-like, referred to as the tissue-nonspecific form of the enzyme, therefore, There are at least four distinct but related alkaline phosphatases: intestinal |
| Immunogen: |
A portion of amino acids 217-246 from human ALPL was used as the immunogen for this Alkaline Phosphatase antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Alkaline Phosphatase antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Alkaline Phosphatase (liver) |
| Short name: |
Alkaline Phosphatase Antibody (liver) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Alkaline Phosphatase |
| Alternative name: |
Alkaline Phosphatase (Antibody to) (liver) |
| Alternative technique: |
antibodies |