| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Akt1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the Akt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Akt1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P31750 |
| Purity: |
Antigen affinity |
| Description: |
Alternatively spliced transcript variants encoding different isoforms have been found for this gene, It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1, It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), [provided by RefSeq], cell survival (anti-apoptosis) and development, epidermal growth factor (EGF), glycogen biosynthesis, inflammatory response, insulin and insulin-like growth factor I (IGF-I), protein synthesis and turn over, such as glucose metabolism, This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3 |
| Immunogen: |
A portion of amino acids 22-49 from the mouse protein was used as the immunogen for this Akt1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Akt1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Akt1 |
| Short name: |
Akt1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
v-akt murine thymoma viral oncogene homolog 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
28426 |
| Gene: |
AKT1S1 |
More about : AKT1S1 |
| Long gene name: |
AKT1 substrate 1 |
| Synonyms gene name: |
AKT1 substrate 1 (proline-rich) |
| Synonyms: |
PRAS40 MGC2865 Lobe |
| Synonyms name: |
40 kDa , proline-rich Akt substrate |
| Locus: |
19q13, 33 |
| Discovery year: |
2004-03-04 |
| GenBank acession: |
BC022241 |
| Entrez gene record: |
84335 |
| Pubmed identfication: |
12524439 |
| RefSeq identity: |
NM_032375 |
| Classification: |
MTOR complex 1 |
| Havana BLAST/BLAT: |
OTTHUMG00000150246 |