| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Akt1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the Akt1 antibody should be determined by the researcher |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P31750 |
| Purity: |
Antigen affinity |
| Description: |
AKT1 and the related AKT2 are activated by platelet-derived growth factor, In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival, It was shown that the activation occurs through phosphatidylinositol 3-kinase, Multiple alternatively spliced transcript variants have been found for this gene, Mutations in this gene have been associated with the Proteus syndrome, Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, The activation is rapid and specific, The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts, and it is abrogated by mutations in the pleckstrin homology domain of AKT1, which then phosphorylates and inactivates components of the apoptotic machinery, RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene |
| Immunogen: |
Amino acids (EDAKEIMQHRFFANIVWQDVYEKKL-mouse) were used as the immunogen for the Akt1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Akt1 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Akt1 |
| Short name: |
Akt1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
v-akt murine thymoma viral oncogene homolog 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
28426 |
| Gene: |
AKT1S1 |
More about : AKT1S1 |
| Long gene name: |
AKT1 substrate 1 |
| Synonyms gene name: |
AKT1 substrate 1 (proline-rich) |
| Synonyms: |
PRAS40 MGC2865 Lobe |
| Synonyms name: |
40 kDa , proline-rich Akt substrate |
| Locus: |
19q13, 33 |
| Discovery year: |
2004-03-04 |
| GenBank acession: |
BC022241 |
| Entrez gene record: |
84335 |
| Pubmed identfication: |
12524439 |
| RefSeq identity: |
NM_032375 |
| Classification: |
MTOR complex 1 |
| Havana BLAST/BLAT: |
OTTHUMG00000150246 |