| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PRMT5 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the PRMT5 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This PRMT5 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O14744 |
| Purity: |
Antigen affinity |
| Description: |
May be part of a pathway that is connected to a chloride current, May play a role in cytokine-activated transduction pathways, May regulate the SUPT5H transcriptional elongation properties, Methylates RPS10, Methylates SUPT5H, Methylates histone H2A and H4 'Arg-3' during germ cell development, Methylates histone H3 'Arg-8', Methylates the Piwi proteins (PIWIL1, Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro, Negatively regulates cyclin E1 promoter activity and cellular proliferation, PIWIL2 and PIWIL4), Plays a role in the assembly of snRNP core particles, Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage, possibly through cytoskeletal rearrangement, such methylation being required for the assembly and biogenesis of snRNP core particles, which may repress transcription, with a preference for the formation of MMA, Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA) |
| Immunogen: |
A portion of amino acids 76-104 from the human protein was used as the immunogen for this PRMT5 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PRMT5 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PRMT5 |
| Short name: |
PRMT5 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PRMT5 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10894 |
| Gene: |
PRMT5 |
More about : PRMT5 |
| Long gene name: |
protein arginine methyltransferase 5 |
| Synonyms gene: |
HRMT1L5 SKB1 |
| Synonyms gene name: |
pombe) , pombe) homolog SKB1 homolog (S, skb1 (S |
| Synonyms: |
SKB1Hs |
| Locus: |
14q11, 2 |
| Discovery year: |
1999-06-18 |
| GenBank acession: |
AF015913 |
| Entrez gene record: |
10419 |
| Pubmed identfication: |
9843966 |
| Classification: |
Protein arginine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000028709 |