| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MGMT |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, FACS |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This MGMT antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P16455 |
| Purity: |
Purified |
| Description: |
Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, This is a suicide reaction: the enzyme is irreversibly inactivated, MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA |
| Immunogen: |
A portion of amino acids 1-30 from the human protein was used as the immunogen for this MGMT antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the MGMT antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MGMT |
| Short name: |
MGMT Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
O-6-methylguanine-Desoxyribonucleic acid methyltransferase (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-methyltransferase activity, MGMT and IDBG-93188 and ENSG00000170430 and 4255, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314, nuclei, this GO :0003677 and DNA binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0003908 and methylated-DNA-[protein]-cysteine S-methyltransferase activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006266 and DNA ligation and biological process this GO :0006281 and DNA repair and biological process this GO :0006306 and DNA methylation and biological process this GO :0006307 and DNA dealkylation involved in DNA repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008168 and methyltransferase activity and molecular function this GO :0009008 and DNA-methyltransferase activity and molecular function this GO :0009636 and response to toxic substance and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0034599 and cellular response to oxidative stress and biological process this GO :0042493 and response to drug and biological process this GO :0043281 and regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045739 and positive regulation of DNA repair and biological process this GO :0051593 and response to folic acid and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071479 and cellular response to ionizing radiation and biological process, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003824 : catalytic activity and also this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity and also this GO :0005509 : calcium ion binding and also this GO :0008168 : methyltransferase activity and also this GO :0009008 : DNA-methyltransferase activity, this GO :0003824 : catalytic activity, this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity, this GO :0005509 : calcium ion binding, this GO :0008168 : methyltransferase activity, this GO :0009008 : DNA-methyltransferase activity, O-6-methylguanine-DNA methyltransferase |
| Identity: |
7059 |
| Gene: |
MGMT |
More about : MGMT |
| Long gene name: |
O-6-methylguanine-DNA methyltransferase |
| Synonyms name: |
methylated-DNA--protein-cysteine methyltransferase |
| Locus: |
10q26, 3 |
| Discovery year: |
1989-10-16 |
| GenBank acession: |
M29971 |
| Entrez gene record: |
4255 |
| RefSeq identity: |
NM_002412 |
| Havana BLAST/BLAT: |
OTTHUMG00000019261 |