| Long name: |
2, 4) Monoclonal Antibody, MGMT (888CT22 |
| Also known as: |
2, 4) Monoclonal Antibody, MGMT (888CT22 |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
MGMT |
| Specificity: |
This is a highly specific antibody against MGMT |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
2, 4, 888CT22 |
| Source: |
Purified His-tagged MGMT protein was used to produced this monoclonal antibody |
| Gene ID number: |
4255 |
| Swiss Prot: |
P16455 |
| Tested Applications: |
IF, IHC-P, WB |
| Recommended dilutions: |
IF(1:25), IHC-P(1:25), WB(1:1000) |
| Cross reactivity: |
Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, This is a suicide reaction: the enzyme is irreversibly inactivated, Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA |
| Purification method: |
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
2, 4) Monoclonal, MGMT (888CT22 |
| Short name: |
2, 4) Monoclonal Antibody, MGMT (888CT22 |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
2, 4) monoclonal (Antibody to), O-6-methylguanine-Desoxyribonucleic acid methyltransferase (888CT22 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-methyltransferase activity, MGMT and IDBG-93188 and ENSG00000170430 and 4255, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314, nuclei, this GO :0003677 and DNA binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0003908 and methylated-DNA-[protein]-cysteine S-methyltransferase activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006266 and DNA ligation and biological process this GO :0006281 and DNA repair and biological process this GO :0006306 and DNA methylation and biological process this GO :0006307 and DNA dealkylation involved in DNA repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008168 and methyltransferase activity and molecular function this GO :0009008 and DNA-methyltransferase activity and molecular function this GO :0009636 and response to toxic substance and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0034599 and cellular response to oxidative stress and biological process this GO :0042493 and response to drug and biological process this GO :0043281 and regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045739 and positive regulation of DNA repair and biological process this GO :0051593 and response to folic acid and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071479 and cellular response to ionizing radiation and biological process, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003824 : catalytic activity and also this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity and also this GO :0005509 : calcium ion binding and also this GO :0008168 : methyltransferase activity and also this GO :0009008 : DNA-methyltransferase activity, this GO :0003824 : catalytic activity, this GO :0003908 : methylated-DNA-[protein]-cysteine S-methyltransferase activity, this GO :0005509 : calcium ion binding, this GO :0008168 : methyltransferase activity, this GO :0009008 : DNA-methyltransferase activity, O-6-methylguanine-DNA methyltransferase |
| Identity: |
7059 |
| Gene: |
MGMT |
More about : MGMT |
| Long gene name: |
O-6-methylguanine-DNA methyltransferase |
| Synonyms name: |
methylated-DNA--protein-cysteine methyltransferase |
| Locus: |
10q26, 3 |
| Discovery year: |
1989-10-16 |
| GenBank acession: |
M29971 |
| Entrez gene record: |
4255 |
| RefSeq identity: |
NM_002412 |
| Havana BLAST/BLAT: |
OTTHUMG00000019261 |