| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
eNos |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This eNos antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29474 |
| Purity: |
Antigen affinity |
| Description: |
NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets, [UniProt], eNos/NOS3 produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway |
| Immunogen: |
A portion of amino acids 1156-1183 from the human protein was used as the immunogen for this eNos antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot the eNos antibody and store frozen at -20 deg, Store at 4oC for up to one month |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
eNos |
| Short name: |
eNos Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
eNos (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7876 |
| Gene: |
NOS3 |
More about : NOS3 |
| Long gene name: |
nitric oxide synthase 3 |
| Synonyms gene name: |
nitric oxide synthase 3 (endothelial cell) |
| Synonyms: |
ECNOS eNOS |
| Synonyms name: |
endothelial nitric oxide synthase |
| Locus: |
7q36, 1 |
| Discovery year: |
1993-08-23 |
| Entrez gene record: |
4846 |
| Pubmed identfication: |
1379542 |
| RefSeq identity: |
NM_000603 |
| Havana BLAST/BLAT: |
OTTHUMG00000158343 |
| Locus Specific Databases: |
The Globin Gene Server |