| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
eNOS |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the eNOS antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This eNOS antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P29474 |
| Purity: |
Antigen affinity |
| Description: |
AKT mediates the activation of eNOS, Fulton et al, Inhibition of the PI3K-AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation, RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells(HUVECs) and human aortic vascular smooth muscle cells(HAOVSMCs) was inversely proportional to that of NOS3AS, The NOS3 gene is mapped on 7q36, Variations in this gene are associated with susceptibility to coronary spasm, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction andplatelet aggregation, also called eNOS, leading to increased nitric oxide production, (1999) concluded the protein is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide, 1, NOS3 (Nitric Oxide Synthase 3) |
| Immunogen: |
Amino acids 144-165 (QAHEQRLQEVEAEVAATGTYQL-human) were used as the immunogen for this eNOS antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the eNOS antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
eNOS |
| Short name: |
eNOS Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
eNOS (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7876 |
| Gene: |
NOS3 |
More about : NOS3 |
| Long gene name: |
nitric oxide synthase 3 |
| Synonyms gene name: |
nitric oxide synthase 3 (endothelial cell) |
| Synonyms: |
ECNOS eNOS |
| Synonyms name: |
endothelial nitric oxide synthase |
| Locus: |
7q36, 1 |
| Discovery year: |
1993-08-23 |
| Entrez gene record: |
4846 |
| Pubmed identfication: |
1379542 |
| RefSeq identity: |
NM_000603 |
| Havana BLAST/BLAT: |
OTTHUMG00000158343 |
| Locus Specific Databases: |
The Globin Gene Server |