| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MITF |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000-2000 |
| Notes: |
Titration of the MITF antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This MITF antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O75030 |
| Purity: |
Antigen affinity |
| Description: |
Alternatively spliced transcript variants encoding different isoforms have been identified, Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes, such as Waardenburg syndrome type 2 and Tietz syndrome, This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features |
| Immunogen: |
A portion of amino acids 1-28 from the human protein was used as the immunogen for this MITF antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the MITF antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MITF |
| Short name: |
MITF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
microphthalmia-associated transcription factor (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006461 and protein complex assembly and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0030154 and cell differentiation and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043010 and camera-type eye development and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0043473 and pigmentation and biological process this GO :0044336 and canonical Wnt signaling pathway involved in negative regulation of apoptotic process and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045670 and regulation of osteoclast differentiation and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046849 and bone remodeling and biological process this GO :0046983 and protein dimerization activity and molecular function, MITF and IDBG-44086 and ENSG00000187098 and 4286, Mitf and IDBG-172461 and ENSMUSG00000035158 and 17342, bHLHe32 and CMM8 and MI and WS2 and WS2A, nuclei, protein dimerization activity, this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006355 and regulation of transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0046983 : protein dimerization activity, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0046983 : protein dimerization activity, 29882 and IDBG-644985 and ENSBTAG00000006679 and 407219, microphthalmia-associated transcription factor |
| Identity: |
7105 |
| Gene: |
MITF |
More about : MITF |
| Long gene name: |
melanogenesis associated transcription factor |
| Synonyms gene: |
WS2A WS2 |
| Synonyms gene name: |
Waardenburg syndrome, type 2A microphthalmia-associated transcription factor |
| Synonyms: |
MI bHLHe32 |
| Synonyms name: |
homolog of mouse microphthalmia |
| Locus: |
3p13 |
| Discovery year: |
1993-10-27 |
| Entrez gene record: |
4286 |
| Pubmed identfication: |
8069297 7874167 7951321 |
| RefSeq identity: |
NM_198159 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000149921 |
| Locus Specific Databases: |
LOVD - Leiden Open Variation Database LRG_776 |