| Long name: |
MITF Primary Polyclonal Antibody |
| Also known as: |
MITF PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
MITF |
| Specificity: |
This is a highly specific antibody against MITF |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
350-400/526 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MITF |
| Gene ID number: |
4286 |
| Swiss Prot: |
O75030 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, Plays a critical role in the differentiation of various cell types, Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1), mast cells, osteoclasts and optic cup-derived retinal pigment epithelium, proliferation and survival, such as BCL2 and tyrosinase (TYR), such as neural crest-derived melanocytes, Transcription factor that regulates the expression of genes with essential roles in cell differentiation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
CMM8, Class E basic helix-loop-helix protein 32, MITF, Microphthalmia-associated transcription factor, WS2, WS2A, bHLHe32, MI |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MITF |
| Short name: |
MITF Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
microphthalmia-associated transcription factor (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006461 and protein complex assembly and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0030154 and cell differentiation and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043010 and camera-type eye development and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0043473 and pigmentation and biological process this GO :0044336 and canonical Wnt signaling pathway involved in negative regulation of apoptotic process and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045670 and regulation of osteoclast differentiation and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046849 and bone remodeling and biological process this GO :0046983 and protein dimerization activity and molecular function, MITF and IDBG-44086 and ENSG00000187098 and 4286, Mitf and IDBG-172461 and ENSMUSG00000035158 and 17342, bHLHe32 and CMM8 and MI and WS2 and WS2A, nuclei, protein dimerization activity, this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006355 and regulation of transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0046983 : protein dimerization activity, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0046983 : protein dimerization activity, 29882 and IDBG-644985 and ENSBTAG00000006679 and 407219, microphthalmia-associated transcription factor |
| Identity: |
7105 |
| Gene: |
MITF |
More about : MITF |
| Long gene name: |
melanogenesis associated transcription factor |
| Synonyms gene: |
WS2A WS2 |
| Synonyms gene name: |
Waardenburg syndrome, type 2A microphthalmia-associated transcription factor |
| Synonyms: |
MI bHLHe32 |
| Synonyms name: |
homolog of mouse microphthalmia |
| Locus: |
3p13 |
| Discovery year: |
1993-10-27 |
| Entrez gene record: |
4286 |
| Pubmed identfication: |
8069297 7874167 7951321 |
| RefSeq identity: |
NM_198159 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000149921 |
| Locus Specific Databases: |
LOVD - Leiden Open Variation Database LRG_776 |