| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SP1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This SP1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P08047 |
| Purity: |
Antigen affinity |
| Description: |
Binds also the PDGFR-alpha G-box promoter, Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, Highly regulated by post-translational modifications (phosphorylations, Implicated in chromatin remodeling, May have a role in modulating the cellular response to DNA damage, Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter, Plays an essential role in the regulation of FE65 gene expression, [UniProt], apoptosis, differentiation and immune responses, glycosylation and acetylation), proteolytic cleavage, sumoylation, Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli |
| Immunogen: |
A portion of amino acids 677-707 from the human protein was used as the immunogen for this SP1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the SP1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SP1 |
| Short name: |
SP1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SP1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11205 |
| Gene: |
SP1 |
More about : SP1 |
| Long gene name: |
Sp1 transcription factor |
| Synonyms name: |
specificity protein 1 |
| Locus: |
12q13, 13 |
| Discovery year: |
1991-06-04 |
| GenBank acession: |
J03133 |
| Entrez gene record: |
6667 |
| Pubmed identfication: |
1662663 |
| Classification: |
Sp transcription factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000170047 |