| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SP1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SP1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P08047 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, By in situ hybridization, Gaynor et al, It belongs to the Sp/KLF family of transcription factors, Matera and Ward(1993) mapped the SP1 gene to 12q13, Segmentation in Drosophila is based on a cascade of hierarchical gene interactions initiated by maternally deposited morphogens that define the spatially restricted domains of gap gene expression at blastoderm, The formation of 7 head segments depends on the function of several genes, The protein is 785 amino acids long, Wimmer et al, with a molecular weight of 81 kDA, (1993) concluded that 12q13, (1993) showed that one of these genes is the Drosophila homolog of the human transcription factor SP1, 1 is the most probable location of the gene, Specificity Protein 1 is a human transcription factor involved in gene expression in the early development of an organism |
| Immunogen: |
An amino acid sequence from the N-terminus of human SP1 (MSDQDHSMDEMTA) was used as the immunogen for this SP1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the SP1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SP1 |
| Short name: |
SP1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SP1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11205 |
| Gene: |
SP1 |
More about : SP1 |
| Long gene name: |
Sp1 transcription factor |
| Synonyms name: |
specificity protein 1 |
| Locus: |
12q13, 13 |
| Discovery year: |
1991-06-04 |
| GenBank acession: |
J03133 |
| Entrez gene record: |
6667 |
| Pubmed identfication: |
1662663 |
| Classification: |
Sp transcription factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000170047 |