| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SREBP2 (SREBF2) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the SREBP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This SREBP2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q12772 |
| Purity: |
Antigen affinity |
| Description: |
Each SREBP consists of three domains, SREBP-1c, SREBP-2 is ubiquitously detected in various tissues, SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway, Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein, Upon cholesterol depletion, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and SREBP-2, and a carboxy-terminal regulatory domain of ~590 amino acids, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism, including SREBP-1a, including an amino-terminal transcription factor domain of ~480 amino acids, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol, SREBPs |
| Immunogen: |
A portion of amino acids 399-427 from the human protein was used as the immunogen for this SREBP2 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the SREBP2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SREBP2 (SREBF2) |
| Short name: |
SREBP2 Antibody (SREBF2) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SREBP2 (Antibody to) (SREBF2) |
| Alternative technique: |
antibodies |
| Identity: |
11290 |
| Gene: |
SREBF2 |
More about : SREBF2 |
| Long gene name: |
sterol regulatory element binding transcription factor 2 |
| Synonyms: |
SREBP2 bHLHd2 |
| Locus: |
22q13, 2 |
| Discovery year: |
1994-11-23 |
| GenBank acession: |
U02031 |
| Entrez gene record: |
6721 |
| Pubmed identfication: |
7903453 |
| RefSeq identity: |
NM_004599 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000151261 |