| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Dnmt3a |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Dnmt3a antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9Y6K1 |
| Purity: |
Purified |
| Description: |
Acts as a transcriptional corepressor for ZBTB18, Can actively repress transcription through the recruitment of HDAC activity, DNA methylation is coordinated with methylation of histones, It modifies DNA in a non-processive manner and also methylates non-CpG sites, May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1, Plays a role in paternal and maternal imprinting, Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites, Required for methylation of most imprinted loci in germ cells, [UniProt], Dnmt3a is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development |
| Immunogen: |
A portion of amino acids 457-486 from the human protein was used as the immunogen for this Dnmt3a antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Dnmt3a antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Dnmt3a |
| Short name: |
Dnmt3a Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Dnmt3a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2978 |
| Gene: |
DNMT3A |
More about : DNMT3A |
| Long gene name: |
DNA methyltransferase 3 alpha |
| Synonyms gene name: |
DNA (cytosine-5-)-methyltransferase 3 alpha |
| Locus: |
2p23, 3 |
| Discovery year: |
1998-07-15 |
| Entrez gene record: |
1788 |
| Pubmed identfication: |
9662389 10433969 |
| RefSeq identity: |
NM_022552 |
| Classification: |
PWWP domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000094777 |
| Locus Specific Databases: |
LRG_459 |