| Antigen detectable with this ELISA kit: |
Apoptosis Inhibitor 5 (API5) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
122ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inhibitor 5 (API5), The concentration of Apoptosis Inhibitor 5 (API5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inhibitor 5 (API5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Apoptosis Inhibitor 5 (API5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Not all receptor antagonist that bind to enzymes are inhibitors, Since blocking an enzyme's activity can kill a pathogen , activator, caspase, esterase inhibitors , lipoprotein, many drugs are enzyme inhibitors, papain, pathway, peptidase, protease , proteinase, trypsin, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme, , Tissue, acrosin, activity, and decreases its , are , bind to enzymes and increase their , enzymatic activity, enzyme activator ligands or agonists , enzyme receptor , imbalance, metabolic , or correct a , or receptor ligands or receptor antagonists that bind to an , proteins |
| Gene target: |
Inhibitor 5 (API5) |
| Short name: |
Apoptosis Inhibitor 5 (API5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
programmed cellular destruction suppressor 5 (apoptosis inhibitor 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AAC-11 and AAC11, API5 and IDBG-40293 and ENSG00000166181 and 8539, API5 and IDBG-636799 and ENSBTAG00000014474 and 538968, Api5 and IDBG-193023 and ENSMUSG00000027193 and 11800, nuclei, poly(A) RNA binding, this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005681 and spliceosomal complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006915 and apoptotic process and biological process this GO :0016020 and membrane and cellular component this GO :0017134 and fibroblast growth factor binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :2000270 and negative regulation of fibroblast apoptotic process and biological process, this GO :0005488 : binding, this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0017134 : fibroblast growth factor binding and also this GO :0044822 : poly(A) RNA binding, this GO :0005515 : protein binding, this GO :0017134 : fibroblast growth factor binding, this GO :0044822 : poly(A) RNA binding, apoptosis inhibitor 5 |
| Identity: |
594 |
| Gene: |
API5 |
More about : API5 |
| Long gene name: |
apoptosis inhibitor 5 |
| Synonyms: |
AAC-11 API5L1 AAC11 |
| Synonyms name: |
API5-like 1 fibroblast growth factor 2-interacting factor 2 migration-inducing protein MIG8 |
| Locus: |
11p12 |
| Discovery year: |
1998-10-06 |
| GenBank acession: |
U83857 |
| Entrez gene record: |
8539 |
| Pubmed identfication: |
9307294 |
| RefSeq identity: |
NM_006595 |
| Havana BLAST/BLAT: |
OTTHUMG00000166395 |